IC50 Calculator
IC50 Calculator
Calculate the half-maximal inhibitory concentration from concentration-response data.
How to use:
Enter drug/inhibitor concentrations and the corresponding response or inhibition values.
Use percentage inhibition for best results. At least 4 valid data points are recommended.
| # | Concentration | Unit | Response / Inhibition (%) |
|---|---|---|---|
| 1 | |||
| 2 | |||
| 3 | |||
| 4 | |||
| 5 |
Calculation Results
Estimated IC50
—
Hill Slope
—
Data Points
—
Four-Parameter Logistic (4PL) Model
Y = Bottom + (Top − Bottom) / [1 + (X / IC50)^HillSlope]
The calculator estimates IC50 by interpolating the concentration-response relationship on a logarithmic concentration scale. For publication-grade analysis, nonlinear regression using a validated statistical package is recommended.