Serial Dilution Calculator

Published August 19, 2026

Serial Dilution Calculator

Calculate concentrations, dilution factors, stock volumes and diluent volumes for a stepwise serial dilution.

Starting Concentration

Concentration Unit

Dilution Factor Per Step

Number of Steps

Total Volume / Tube

Volume Unit

Animation Speed

Display Precision

Initial Concentration

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Final Concentration

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Cumulative Dilution

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Final Dilution Factor

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Serial Dilution Animation

Concentration Profile

Relative concentration

Dilution Results

Step	Starting Conc.	Dilution Factor	Final Conc.	Cumulative DF	Sample Volume	Diluent Volume

Formula: C₂ = C₁ ÷ DF. For a dilution factor DF, the sample volume is V_sample = V_total ÷ DF and the diluent volume is V_diluent = V_total − V_sample. In a serial dilution, each step uses the concentration from the previous step.