HPLC Dilution Calculator

Published August 19, 2026

HPLC • Analytical Preparation Tool

Calculate the exact volume of stock solution and diluent required to prepare your target HPLC sample using the fundamental dilution equation.

C₁V₁ = C₂V₂

Solution Parameters

Stock concentration (C₁)

Concentration of your stock solution

Desired concentration (C₂)

Target concentration for HPLC analysis

Final volume (V₂)

Total volume of prepared sample

Dilution factor

Optional — calculated automatically if blank

50%

75%

100%

Add stock → add diluent → mix

Stock Take calculated V₁

Dilute Add diluent

Mix Make to final volume

Stock volume required (V₁)

—

Enter values above to calculate.

Diluent volume —

Dilution factor —

Stock fraction —

Final volume —

C₁V₁ = C₂V₂